ReCIVA FAQ

Frequently Asked Questions

Is ReCIVA CE marked?

Yes.

What sample tubes can be used with ReCIVA?

ReCIVA is designed and calibrated for use with Breath Biopsy Cartridges. Use of other tubes may affect the volume of breath captured. 

Can Breath Biopsy Cartridges be analyzed with standard GC/MS?

Yes, Breath Biopsy Cartridges can be analyzed using GC-MS or GC-FAIMS. See below for further guidance on analysis.

What guidance can you give on analysis of the Breath Biopsy Cartridges?

In the selection of the sorbent bed there is a tradeoff between retention of lighter VOCs and water. Higher levels of water can result in siloxanes in GC-MS analysis. We have several measures in place to minimize water content loaded onto the GC: 1) Run a 3 min purge on the instrument prior to desorption. 2) Use a less polar column (we use a VF-5ms column with a 0.25um inner thickness). 3) Use a higher temperature (we use 20C) so that water is not retained on the cold trap while the other compounds are. 4) Running a higher split onto the GC (we currently use 1:2 with GC-TOF and 1:1 with GC-FAIMS. 5) It may also be possible to collect double the sample volume and double the split to load the same amount of sample and half of the water content compared to your current method. The hydrophobic nature of the sorbent means that VOCs would be concentrating while water is breaking through, so it may help to collect more and split more so that more water can be removed without significantly impacting on sensitivity

If you don't want to get four samples per patient, is there any way of covering the holes in the mask?

If you want to run less than four samples per patient, please contact us. We can provide you with solid rods to replace the tubes you’re not fitting. You can then configure the software to tell it how many tubes you are using. Note that you need to block both the hole in the mask and the inlet to ReCIVA. 

What is the stability of samples after collection?

Unnecessary storage of samples should be avoided, but current evidence suggests that the effects of delaying analysis are minimized by keeping tubes well sealed and at low temperatures.

For longer term storage we recommend capping tubes using Swagelok tube fittings:

2 x Swagelok ¼” tube fitting brass nuts, part no: B-402-1

2 x Swagelok ¼” tube fitting brass caps, part no: B-400-C

2 x Swagelok ¼” tube fitting PTFE Ferrule Set (1 Front/1 Back), part no: T-400-SET

Note that caps on sorbent tubes should be re-tightened after they have equilibrated with their cold storage conditions, this is particularly important when storing in the freezer.

The stability of compounds in stored tubes will vary between species and on which sorbent(s) they are adsorped on. Evidence suggests that minimal changes are observed in Tenax TA (SW Harshman et al., Storage stability of exhaled breath on Tenax TA, Journal of Breath Research, Volume 10, Number 4, 2016) and Tenax GR (MP van der Schee et al., Effect of transportation and storage using sorbent tubes of exhaled breath samples on diagnostic accuracy of electronic nose analysis, Journal of Breath Research, Volume 7, Number 1, 2013) tubes refrigerated for a period of 2 weeks. Storage at -80C for up to 1.5 months has been shown to result in no discernible changes to samples kept on Tenax TA/Carbograph multi-bed tubes (S Kang and CL Paul Thomas, How long may a breath sample be stored for at  −80 °C? A study of the stability of volatile organic compounds trapped onto a mixed Tenax:Carbograph trap adsorbent bed from exhaled breath, Journal of Breath Research, Volume 10, Number 2, 2016).

Can I use sorbent tubes instead of Breath Biopsy Cartridges?

The ReCIVA Breath Sampler is calibrated for the flow possible through Breath Biopsy Cartridges. The pressure drop through different sorbent tubes may be higher/lower, and this will affect the accuracy of breath collection volumes set in the BSC software. For example, a target flow of 200mL/min with tubes with high flow resistance might only collect at 160mL/min (i.e. 20% lower total breath volume). Please contact us if you want to use a different type of collection tube.

Note that using different sorbents will also affect the range of VOCs sampled, and potentially change the amount of water captured during collection.

I was expecting to find a CD containing software for installation. Why isn’t there one in the box?

Software for the ReCIVA is supplied on a USB drive that is shipped with the ReCIVA unit. If you have not received a USB drive or require the software in a different format for installation please contact us via our support portal.

I need additional head straps for my ReCIVA, can you provide extra ones?

Yes, please contact us via our support portal for details.

Can ReCIVA masks be re-used?

No. To minimize cross contamination and ensure infection control, masks must not be re-used.

What is the material of the ReCIVA mask and does it contain Latex?

The mask is made of silicone and does not contain any Latex.

Does the CASPER Portable Air Supply require a gas supply?

The CASPER Portable Air Supply pumps and filters ambient room air so it does not require a separate supply of gas.

How often does the air scrubber incorporated into the CASPER Portable Air Supply need to be replaced?

The scrubber should be replaced once a year (or every 450 hours use whichever comes first).

What is the material/specs of the clear tubing used for the CASPER Portable Air Supply?

The clear tubing is Tygon E-3603, the tubing must be baked prior to use to minimize the release of unwanted VOCs. Please contact us if you need more information.

Does the CASPER Portable Air Supply include particulate filters?

The CASPER scrubber unit contains a 3-stage filter: 100 micron, 40 micron and HEPA. There is also a final AcroPak filter on the output, which can be purchased from VWR here.

Do you have a video guide to using ReCIVA?

Yes we do! Take a look here: RECIVA VIDEO GUIDE

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