Stamatios Giannoukos at the Breath Biopsy Conference 2022
(20 mins) ADVANCES IN TECHNOLOGIES IN BREATH ANALYSIS: Advances in breath metabolomics using secondary electrospray ionization high-resolution mass spectrometry
On-line breath analysis based on secondary electrospray ionization-high-resolution mass spectrometry (SESI-HRMS) has served as a sensitive method for untargeted metabolomics and biomarker discovery. The advantageous characteristics of this technique make it an ideal chemical analysis tool for clinical investigations as well as different health-related fields, including nutritional science. In addition, the calibration of mass spectrometers is of major importance, especially for quantitative measurements. Quantification of exhaled breath metabolites is associated with measuring the device’s analytical performance, accuracy, reproducibility, reliability, and stability. Herewith, we report on a range of recent improvements for breath metabolomics using SESI-MS including optimization of the softness of the method, sensitivity enhancement, and quantification of volatile biomarkers of health and nutritional interest.
Stamatios Giannoukos is a Senior Scientist at the Department of Chemistry and Applied Biosciences at the ETH Zurich. He received an MSc degree in Chemical Engineering from the National Technical University of Athens (Greece) and a Ph.D. degree in Electrical Engineering and Electronics from the University of Liverpool, (UK). Previously, he was a PSI- Marie Skłodowska-Curie Fellow at the Laboratory of Atmospheric Chemistry at the Paul Scherrer Institute in Switzerland.
He is specialized in ambient ionization, portable and benchtop mass spectrometry, volatile organic compounds (VOCs), and in-field chemical analysis (outside the lab). His current work includes the molecular investigation and characterization of the human exhaled breath metabolome in response to nutrition (NutriExhalome). He focuses on real-time breath analysis using secondary electrospray ionization (SESI) high-resolution mass spectrometry (HR-MS) and investigates the effect of certain foods and nutrients on the human postprandial exhalome and metabolism. He also works on the characterization of bovine breath for animal health and welfare monitoring, animal communication, and the investigation and characterization of microbial communities’ volatilomic and metabolomic profiles. He also works with tandem MS for compound identification, develops methodologies for the quantification of molecules in the gas phase, and chemometric tools/strategies to handle high-dimensional data from balanced and unbalanced multifactorial designs.
Giannoukos has worked extensively in the development of various MS methodologies for detecting VOCs in homeland security, forensics, search and rescue, precision agriculture, environmental, and nuclear applications. He has also developed apparatuses that can produce gaseous standards with high precision for calibration purposes and he has investigated molecular communications in macroscale environments using MS. He has acted as a consultant for several international companies and has entrepreneurial experience in the MS market. He is the author of more than 35 peer-reviewed journal publications and more than 60 conference publications
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