ReCIVA Breath Sampler
A reliable and reproducible way to capture VOC biomarkers in breath samples
- Flexible breath collection - allows selection of different volumes and fractions (end-tidal, inhaled, bronchial) of breath
- Exhaled breath VOCs can either be sent directly to an analytical instrument for analysis in real time or collected on two pairs of sorbent tubes for later analysis
- CE marked and in use in over 60 academic research groups and clinical labs around the world
Developed in conjunction with a multi-disciplinary group of researchers, ReCIVA provides an out-of-the-box means of reliably and reproducibly capturing breath samples for analysis. This allows researchers to focus their efforts on analysing the captured breath. The system is currently being deployed as part of Owlstone's LuCID lung cancer detection project.
Depending on the particular disease under investigation, the VOCs of interest will be present in different breath fractions. Pressure and CO2 sensors in ReCIVA provide real-time monitoring of the patient's breathing, allowing the relevant fractions to be selected. Fast sensor response ensures the required breath fraction is accurately collected, and the sampler is constructed using inert materials to prevent contamination of the breath sample by exogenous VOCs.
Different breath fractions can be stored independently on two pairs of sorbent tubes, which can be analysed immediately or stored for later use. Sorbent tubes ensure reproducible collection of volatile biomarkers, unlike other commonly used breath collection media.
The ReCIVA has been designed with patient comfort and safety as top priorities. Requiring only simple tidal breathing and minimal effort, patient comfort is guaranteed whilst using the ReCIVA breath sampler. Built using easily cleaned, high quality inert materials the ReCIVA prevents cross contamination and ensures patient safety by incorporating a disposable, single use mask and an integral filter.
Flexible breath collection
Allows selection of different volumes and fractions (end-tidal, inhaled, bronchial) of breath
Repeatable and reproducible sampling
Fast sensor response ensures the required breath fraction is accurately collected
High patient safety and comfort
Simple tidal breathing is straightforward for patients to perform
Direct or delayed analysis of samples
Sorbent tubes can be analysed immediately or stored. Compatible with platforms including GC-FAIMS and GC-MS (minor modifications to ensure compatibility)
Compatible with supplemental oxygen
Additional oxygen can be supplied pre-mixed to patients via air supply port
no risk of cross-contamination
Disposable single-use masks and the integral filter prevent any cross-contamination from occurring
How it works
Designed in collaboration with over 100 leading breath diagnostics researchers
Reproducibility is key for any analytical technique, and standardisation of sample collection is a vital step in guaranteeing the comparability of results from different methods of chemical analysis. The Breathe Free Consortium is a multi disciplinary expert discussion forum that was formed by Owlstone Medical to develop a standardised design for a breath sampler that will provide a benchmark for the collection of breath samples for chemical analysis and medical diagnosis. The ReCIVA is a CE marked and validated, commercially available sampler manufactured by Owlstone Medical.
Monitor patient breathing patterns in real time
Using internal, fast response CO2 and pressure sensors ReCIVA can monitor patient breathing patterns in real time (see left). This allows it to track when particular parts of the patient's breath (e.g. end-tidal or bronchial fractions) are being exhaled. The supplied software can then be used to turn the sampling pumps on and off at the necessary time to collect the required fraction.
Getting the full picture through a straightforward software interface
Using the straightforward software interface (see right), the following fractions can be selected: upper airways only, lower airways only, upper and lower airways, whole breath (including mouth air) and upper airways including mouth air. Selected volumes and fractions of exhaled breath VOCs can either be sent directly to an analytical instrument for analysis in real time or collected on two pairs of Tenax (or other) sorbent packed tubes for later analysis. Each pair of sorbent tubes is gated independently, allowing different breath fractions to be sampled in a single collection event.
ReCIVA Paediatric Masks
The ReCIVA breath sampler is available with adult and child-sized masks, both of which have been CE marked. The paediatric mask extends the range of ages that can be sampled down to around 5 years old. The non-invasive nature of breath testing makes it a particularly attractive option for use with younger patients, who may be reluctant to give e.g. a blood sample. The masks have opened up a whole new range of research possibilities, and are already being deployed in studies of childhood asthma.
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Real time monitoring or archival breath sample collection
ReCIVA can be used for both real time monitoring of breath chemical composition and for archival collection of breath samples onto sorbent tubes for later analysis. ReCIVA is ideal for use in conjunction with techniques such as SIFT-MS (Selected-ion flow-tube mass spectrometry) and can be easily configured to supply defined breath volumes and fractions, allowing straightforward comparison between, for instance, end-tidal and bronchial breath fractions. Concentrations of endogeneous VOCs are up to three times higher in gas from the alveoli (the end-tidal fraction) than mixed expiratory gas and alveolar samples are also less prone to contamination. The use of sorbent tubes allows sample preconcentration, allowing detection of lower concentration analytes.