ReCIVA® Breath Sampler
A reliable and reproducible way to capture VOC biomarkers in breath samples
- Exhaled breath Volatile Organic Compounds (VOCs) are collected on a Breath Biopsy Cartridge for later analysis
- High patient safety and comfort
- CE marked and in use in over 100 academic research groups and clinical labs around the world
Developed in conjunction with a multi-disciplinary group of researchers, the ReCIVA Breath Sampler provides an out-of-the-box means of reliably and reproducibly capturing breath samples for analysis. This allows researchers to focus their efforts on analyzing the captured breath. The system is currently being deployed as part of Owlstone Medical's LuCID Clinical Trial for lung cancer detection.
Depending on the particular disease under investigation, the VOCs of interest could be present in different breath fractions. Pressure and CO2 sensors in ReCIVA provide real-time monitoring of the patient's breathing, allowing the relevant fractions to be selected. Fast sensor response ensures the required breath fraction is accurately collected, and the sampler is constructed using inert materials to minimize contamination of the breath sample by exogenous VOCs.
Different breath fractions can be stored independently within the Breath Biopsy Cartridge, which can be analyzed immediately or stored for later use. The Breath Biopsy Cartridge ensures reproducible collection of volatile biomarkers, unlike other commonly used breath collection media.
The ReCIVA Breath Sampler has been designed with patient comfort and safety as top priorities. Requiring only simple tidal breathing and minimal effort, high patient comfort has been reported whilst using the ReCIVA Breath Sampler. Built using easily cleaned, high quality inert materials the ReCIVA minimizes cross contamination and maximizes patient safety by incorporating a disposable, single use mask and an integral filter.
Repeatable and reproducible sampling
Ideal for longitudinal studies measuring breath VOCs over time
High patient safety and comfort
Simple tidal breathing is straightforward for patients to perform
Pre-concentrates samples for later analysis
Breath Biopsy Cartridges can be analyzed immediately or stored
Compatible with gas chromatography
Compatible with platforms including GC-FAIMS and GC-MS (minor modifications to ensure compatibility)
Flexible breath collection
Allows selection of different volumes and fractions (end-tidal, inhaled, bronchial) of breath
Disposable single-use masks and integral filter minimize risk of cross-contamination
How it works
Reproducibility is key for any analytical technique, and standardization of sample collection is a vital step in maximizing the comparability of results from different methods of chemical analysis. The Breathe Free Consortium is a multi disciplinary expert discussion forum that was formed by Owlstone Medical to develop a standardized design for a breath sampler that will provide a benchmark for the collection of breath samples for chemical analysis and medical diagnosis. The ReCIVA is a CE marked and validated, commercially available Breath Sampler manufactured by Owlstone Medical. ReCIVA enables robust, reproducible breath sampling, ideal for longitudinal monitoring of breath VOCs over time.
Using internal, fast response CO2 and pressure sensors ReCIVA can monitor patient breathing patterns in real time (see left). This allows it to track when particular parts of the patient's breath (e.g. end-tidal or bronchial fractions) are being exhaled. The supplied software can then be used to turn the sampling pumps on and off at the necessary time to collect the required fraction.
Using the straightforward software interface (see right), different breath fractions can be selected. Selected volumes and fractions of exhaled breath VOCs are collected on a Breath Biopsy Cartridge for later analysis. Breath sample replicates and/or different breath fractions can be obtained in a single collection event.
The ReCIVA Breath Sampler is used in conjunction with CASPER Portable Air Supply, designed to improve breath sampling by reducing the abundance of VOCs in inhaled air.
CASPER Portable Air Supply uses a mains-operated air pump which takes ambient air and passes it through an air filter unit before supplying it to ReCIVA. The subject breathes in filtered air, reducing the level of background VOCs from the surroundings that are present in the collected sample.
CASPER is designed for portability and ease of use. Its features include:
- Integrated pump and filter unit
- Air flow-rate designed for subject comfort
- Lift-in/Lift-out filter cartridges
- Remaining filter lifetime indicator lights
- Air temperature and flow sensors
- Easy fitting air tube attachment
- Error warning lights and sounds
Asthma, Lung Cancer, COPD, Inflammatory Disease, Respiratory Disease, Tuberculosis
Breath Biopsy Services
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Breath Biopsy Kits
From collection of high quality breath samples, to discovery and validation of VOC biomarkers
ReCIVA is used to collect breath samples onto Breath Biopsy Cartridges for later analysis. ReCIVA is ideal for use in conjunction with techniques such as GC-MS (gas chromatography-mass spectrometry) and GC-FAIMS (gas chromatography field asymmetric ion mobility spectrometry), and can be easily configured to supply defined breath volumes and fractions, allowing straightforward comparison between, for instance, end-tidal and bronchial breath fractions. Concentrations of endogeneous VOCs are up to three times higher in gas from the alveoli (the end-tidal fraction) than mixed expiratory gas and alveolar samples are also less prone to contamination. The use of Breath Biopsy Cartridges allows sample pre-concentration, enabling even low concentration analytes to be detected.
Owlstone Medical's Products and Services are for research use only. Not for use in diagnostic procedures.